Specificity, efficiency, and fidelity of PCR.

Specificity, efficiency, and fidelity of PCR.

Center for Environmental Health Sciences and Division of Toxicology, Whitaker College of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 The efficacy of PCR is measured by its specificity, efficiency (i.e. yield), and fidelity. A highly specific PCR will generate one and only one amplification product that is the intended target sequence. Mo...

Fidelity of PCR

Center for Environmental Health Sciences and Division of Toxicology, Whitaker College of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139 The efficacy of PCR is measured by its specificity, efficiency (i.e. yield), and fidelity. A highly specific PCR will generate one and only one amplification product that is the intended target sequence. Mo...

Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity

1 Key Laboratory of Subtropical Agriculture & Environment of the Ministry of Agriculture, Huazhong Agricultural University, Wuhan 430070, China; 2 School of Medicine, Jianghan University, Wuhan 430056, China; 3 Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education, Jianghan University, Wuhan 430056, China; 4 State Key Laboratory of Environmental Chemistry and ...

DNA Synthesis Efficiency and Fidelity Mechanisms

The burden of high accuracy synthesis in DNA replication and repair falls in large part on DNA polymerases which incorporate nucleotides to the primer strand complementary to the template bases. Since mutations stemming from polymerase errors can result in permanent genomic change that may lead to human diseases like colon, skin, or lung cancer and premature aging, understanding fidelity mechan...

Specificity and performance of diagnostic PCR assays.